Metabolite extraction

We will not go into the details of quenching, metabolite extraction or choice of mass spectrometry platform here (as to some extent they will be specific to the organism and/ or tissue involved and what questions you are trying to answer).

These references may be of use in comparing methods and making decisions (while they are focussed mostly on plants, much of the information is relevant to other complex biological samples):

  • Jorge TF, Mata AT, António C. (2008). Mass spectrometry as a quantitative tool in plant metabolomics. Philos Trans A Math Phys Eng Sci.374(2079):20150370. doi: 10.1098/rsta.2015.0370
  • Pezzatti J., Boccard J., Codesido S., Gagnebin Y., Joshi A., Picard D., et al. (2020). Implementation of liquid chromatography-high resolution mass spectrometry methods for untargeted metabolomic analyses of biological samples: a tutorial. Anal. Chim. Acta 1105 28–44. doi: 10.1016/j.aca.2019.12.062
  • Villate, A., San Nicolas, M., Gallastegi, M., Aulas, P.-A., Olivares, M., Usobiaga, A., Etxebarria, N., Aizpurua-Olaizola, O. (2021) Metabolomics as a Prediction Tool for Plants Performance under Environmental Stress. Plant Sci. 303: 110789 doi: 10.1016/j.plantsci.2020.110789